sop for centrifuge

SOP for Laboratory Instrument Qualifiion - Pharma …

2020-3-4 · After completion of IQ User shall prepare a draft SOP, after completion of OQ final SOP shall be prepared based on the qualifiion study for the regular usage and performance qualifiion. PQ shall be perform by operating the instrument as per final SOP. The in-house IQ, OQ and PQ protocol shall be prepared (if required).

Safe Operating Procedure - University of Nebraska–Lincoln

2021-5-3 · centrifuge be opened any sooner than 20 minutes after the rotor has come to a complete stop to allow sufficient time for aerosols to settle. General centrifuge decontamination procedures can be found in the EHS SOP, Biological Decontamination of Laboratory Equipment • If the material involved was a radioactive material, promptly contact EHS. A

Centrifuge Standard Operating Procedures

2017-3-6 · {Open the lid of the centrifuge and check for condensation. If the centrifuge is wet and/or dirty, clen it out including the spindle. {Check that the main large seal on the top of the centrifuge is clear of obstructions. {Using the OVERTEMP knob, set the red bar of the temperature meter well above the aient temperature. {Turn the POWER switch ON.

Centrifuge Safety – Stanford Environmental …

The following information may be integrated into a lab-specific standard operating procedure (SOP) for centrifuge use. Preventive Maintenance. Establish a preventive maintenance schedule: Including regular cleaning of centrifuge …

6 Safety Tips for Operating a Centrifuge - Lab Manager

There are a few important guidelines for operating a centrifuge, even a small one. Following them can prevent damage to the centrifuge and possible serious injury to you and others. 1. The work surface must be level and firm. Do not use the centrifuge on an uneven or slanted work surface. 2. Balance the tubes in the rotor!

SOP.008 Operation of the Fixed Angle Centrifuge

2020-8-11 · SOP.008 Operation of the Fixed Angle Centrifuge Updated June 16, 2020 - Page 1 of 2 Purpose The purpose of this SOP is to provide instruction in the proper use of the fixed angle centrifuge. Materials 1. 70% ethanol 2. Kimwipes Methods 1. Select the appropriate rotor for the run. Wipe down the rotor and centrifuge chaer with 70% ethanol.

How to Use a Centrifuge: 14 Steps (with …

2020-11-3 · A centrifuge is a type of research equipment that spins a liquid suspension at high rotation rates to separate it into distinct layers based on …

SOP.008 Operation of the Fixed Angle Centrifuge

2020-8-11 · SOP.008 Operation of the Fixed Angle Centrifuge Updated June 16, 2020 - Page 2 of 2 14. Use the log sheets to report run information including your name, lab name, rotor used, speed, run start time and estimated run time. 15. Once the centrifuge is done running and has come to a complete stop, the chaer door will unlock.

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SOP for Cleaning of Centrifuge : Pharmaceutical Guidelines

2022-4-3 · Head of the Department 5.0 PROCEDURE 5.1 Switch ‘OFF’ the instrument. 5.2 Switch ‘OFF’ the ‘Mains.’ 5.3 Clean the outer surface of the instrument with dry cotton cloth. 5.4 Clean the centrifuge machine disc with cotton cloth. 5.5 After completion of each analysis, wash the centrifuge tube as per glassware cleaning S.O.P.

SOP for Thawing, Culturing, and Freezing Human iPSCs …

2016-8-11 · Centrifuge at 200 g for 5 minutes and discard the washing supernatant. 7. Resuspend the cells in 2 ml of mTeSR medium plus ROCK inhibitor. 8. Aspirate excess Matrigel from well surfaces and plate cells into 1 well of a six well plate. Incubate cells …

SOP.008 Operation of the Fixed Angle Centrifuge

2020-8-11 · SOP.008 Operation of the Fixed Angle Centrifuge Updated June 16, 2020 - Page 1 of 2 Purpose The purpose of this SOP is to provide instruction in the proper use of the fixed angle centrifuge. Materials 1. 70% ethanol 2. Kimwipes Methods 1. Select the appropriate rotor for the run. Wipe down the rotor and centrifuge chaer with 70% ethanol.

SOP - Centrifuge Safety - Bridges Lab Protocols

2019-4-29 · Supersedes: SOP-CS-01.1 Original Date: Deceer 1, 2017 Revised: May 1, 2019 Pages 1 of 9 Issued by: Director, Health Sciences Research . 1.0 POLICY. This procedure describes the standard operation for using the aient and refrigerated centrifuges loed in the Research Centrifuge Room (Connell 4, Room 2-4-041) in the

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SOP CRF.L109 V1.3 Use of the Refrigerated Centrifuge

2014-1-3 · SOP History (archived date) V1.2 (01/11/2010) reviewed by John Humphreys, V1.1 (01/11/2010); V1.0 (03/03/2010) SOP Nuer CRF.L109 Created by Emma Goodwin Use of the Refrigerated Centrifuge This SOP has been written to give general guidance to study personnel on how to use a centrifuge. There are no Good Laboratory Practice Guidelines

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SOP CRF.L109 V1.3 Use of the Refrigerated Centrifuge - …

2014-1-3 · SOP History (archived date) V1.2 (01/11/2010) reviewed by John Humphreys, V1.1 (01/11/2010); V1.0 (03/03/2010) SOP Nuer CRF.L109 Created by Emma Goodwin Version 1.3 Date 30/10/2013 Review Date October 2013 Related SOPs 1 CRF.L105 Approved by Alison Jenkins SOP CRF.L109 Version 1.3 Use of the Refrigerated Centrifuge 30/10/2013 Page 1 of 6

Safe Operating Procedure - University of Nebraska–Lincoln

2021-5-3 · centrifuge be opened any sooner than 20 minutes after the rotor has come to a complete stop to allow sufficient time for aerosols to settle. General centrifuge decontamination procedures can be found in the EHS SOP, Biological Decontamination of Laboratory Equipment • If the material involved was a radioactive material, promptly contact EHS. A

LacCore SOP Collection Pollen Preparation Procedure

Centrifuge SOP for proper use, care, and maintenance. You are responsible for following the information in the Centrifuge SOP. It is essential that the centrifuge buckets be …

6 Safety Tips for Operating a Centrifuge - Lab Manager

There are a few important guidelines for operating a centrifuge, even a small one. Following them can prevent damage to the centrifuge and possible serious injury to you and others. 1. The work surface must be level and firm. Do not use the centrifuge on an uneven or slanted work surface. 2. Balance the tubes in the rotor!

SOP for Centrifuge Machine : Pharmaceutical Guidelines

2022-4-12 · 5.1.1 Ensure that the instrument is clean & free from dust. 5.1.2 Ensure that all the knobs are in normal position. 5.1.3 Open the upper lid by releasing the lock and lifting it up. 5.1.4 Place the centrifuge tubes in the compartment provided for it. 5.1.5 The compartment is designed in such a way to place sixteen centrifuge tubes at a time.

SOP on Operation and Cleaning of Centrifuge - Pharma

2022-4-10 · This SOP describes the procedure for operation and cleaning of Centrifuge. Procedure: Operation Connect the three pin plug with the main supply. To ensure that the rotor has been correctly loaded with its components of tube and buckets, the centrifuge can be started by closing its lid. Now move the speed control knob clock wise from “0” position.

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Centrifuge Safety SOP-CS-01 - kingstonhsc.ca

2018-1-8 · Centrifuge Safety SOP-CS-01 • Ensure the lid remains closed; do not disturbthe centrifuge for 30 minutes after the rotor has stopped to allow aerosols to settle. • PPE MUST be worn before proceeding to the next step. • Obtain Oxivir® solution and Oxivir® wipes loed in the cabinet under the dirty sink.

cGMP and Regulatory Updates: SOP - OPERATION AND …

2008-7-27 · This sop covers operation procedure and calibration for centrifuge apparatus and this sop is applicable to Quality control department. 3.0 RESPONSIBILITY Officer / Executive. 4.0 ACCOUNTABILITY Department Head 5.0 PROCEDURE 5.1 PROCEDURE FOR GENERAL CLEANING. 5.1.1 Clean the instrument with dry cloth from inside chaer and outside of the

General Centrifuge Safety - University of British Coluia

2016-5-31 · Doc #: UBCV-RMS-OHS-SOP-14-002 Title: General Centrifuge Safety Page 2 of 4 Fill out the log each time the instrument is used, Report damage to centrifuge or rotor to the laboratory supervisor so action in terms of repair or de-rating may be performed. After the training takes place, the training must be documented in accordance to

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