microcentrifuge tube vector

Addgene: Protocol - pLKO.1 – TRC Cloning Vector

The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community. Please cite Moffat et al., Cell 2006 Mar; 124 (6):1283-98 (PubMed PMID 16564017) in

Micro Centrifuge Tube

Brown Microcentrifuge Tubes. • USP VI grade polypropylene material, free from heavy metal. • RCF: 30,000 xg. • Special safelock cap design provides secure lid sealing. • Clear graduations and frosted marking area. • Easy opening and closing. • Autoclavable. • RNase free, endotoxin level less than 0.1EU. • Both sterile and non

Vials, Specimen Cups, Microcentrifuge Tubes and Storage

The Floating Tube Rack has the capacity to hold 20 (round) and 16 (square) microcentrifuge tubes (1.5 – 2.0ml). The unique locking tabs keep the tubes secure while immersed. The microcentrifuge tubes can be easily removed by pushing the rack down onto the bench top surface. Polypropylene, microwave transparent.

Microcentrifuge Tube Selector Tool | Thermo Fisher …

Microcentrifuge Selector Tool. Select the microcentrifuge tube to fit your appliion from the filters below. Additionally, you can take the time to review our available microcentrifuge tubes as well. View Compare List. ×.

Microcentrifuge Tubes Eppendorf Tubes Micro Tubes

Microcentrifuge Tubes. Eppendorf Microcentrifuge Tubes. Centrifuge Tubes. Conical Tubes. PCR Tubes. PCR Strip Tubes. Cryogenic Vials. Screw Cap Tubes. Culture Tubes. Cluster Tubes. Chromatography and Scintillation Vials. Cuvettes. Tube Racks and Holders

Microcentrifuge Tubes - Scientific Commodities, …

MICROCENTRIFUGE TUBES. These 1.5 ml polypropylene tubes are leak tested to 20,000 RCF for 20 minutes at 20C with an aqueous solution. The attached lid provides a leak proof seal. The lids can be easily pierced and have a frosted …

Expression and Purifiion of Recoinant Proteins in

2017-6-2 · Add to a microcentrifuge tube: 100 ng of the PCR product in 1–5 μl TE or H 2 O, 1.3 μl of 150 ng/μl pDONR vector DNA, and enough TE to bring the total volume to 12 μl. Mix well. Mix well. Thaw BP Clonase II enzyme mix on ice (2 min) and then vortex briefly (2 s) twice ( …

GEB Microcentrifuge Tube 1.5mL,EP_-

2016-1-7 · GEB Microcentrifuge Tube : Gene Era Biotech microcentrifuge tubes feature extra thick and durable walls which withstand centrifugation up to 20,000 RCF. Our innovative snap-lock cap and 90° hinge design assures an efficient seal while using next-to …

Microcentrifuge Tubes | Thermo Fisher Scientific …

Microcentrifuge Tubes | Thermo Fisher Scientific - US Microcentrifuge Tubes Prepare, transfer and store samples securely Whether you''re preparing, transferring or storing samples, these multipurpose microcentrifuge tubes and …

Microcentrifuge tube-PCR rack, 80 well, reversible

Microcentrifuge tube-PCR rack, 80 well, reversible PCR tube racks rack PCR tubes tube racks : Perfect for benchtop use or freezer storage, this compact, double-sided polypropylene rack accommodates 0.2 mL thin-walled PCR

T-vector - - -

Discard the supernatant and wash the DNA with 70% Ethanol again. 22. Invert the microcentrifuge tubes over paper towels and allow the DNA pellet to air dry for a couple of minutes. 23. Dissolve the DNA pellet in 1X TE. 24. For ligation reactions, use approximately 25 to 50 ng of T-Vector (see Hint #1). Solutions.

Quick Ligation Protocol (M2200) - NEB

1. Set up the following reaction in a microcentrifuge tube on ice. (Quick Ligase should be added last. Note that the table shows a ligation using a molar ratio of 1:3 vector to insert for the indied DNA sizes.) Use NEBiocalculator to calculate molar ratios.

Microcentrifuge Tubes - Accumax

2022-4-11 · Microcentrifuge Tubes. Accumax offers high-quality Microcentrifuge tubes made from polypropylene raw material which offers comfort and dependability for storage, Isolation, centrifugation, and Sample preparation purposes.

Microcentrifuge Tubes Eppendorf Tubes Micro Tubes …

Microcentrifuge Tubes. Eppendorf Microcentrifuge Tubes. Centrifuge Tubes. Conical Tubes. PCR Tubes. PCR Strip Tubes. Cryogenic Vials. Screw Cap Tubes. Culture Tubes. Cluster Tubes. Chromatography and Scintillation Vials. Cuvettes. Tube Racks and Holders

1.5mL Microcentrifuge Tubes - BrandTech …

Description. BRAND ® microcentrifuge tubes fit most common rotors and may be spun up to 30,000 RCF (g) (10,000 x g for 0.5mL; 20,000 x g for 1.5mL; 30,000 x g for 1.5mL and 2.0mL with lid closure at 20°C for 20 min). Manufactured from …

Axygen MaxyClear SnapLock Microcentrifuge …

2022-4-14 · SnapLock microcentrifuge tubes have frosted cap surfaces and frosted side panels, ideal for labeling. Each cap features a piercing port in the center of the cap. Microcentrifuge tubes with no caps are available for some …

0.5mL Microcentrifuge Tube - GenFollower

0.5mL Microcentrifuge Tube GenFollower online 2021-07-16T03:20:22+00:00. 0.5ml Polypropylene Microcentrifuge Tubes. Made of original polypropylene (PP), autoclavable. One of the foremost OEM manufacturers, GenFollower offers …

BRAND ® microcentrifuge tube, 0.5 mL with lid

BRAND ® microcentrifuge tube, 0.5 mL with lid BRAND ® ,0.5 mL, BR780536 500 EA, clear polypropylene clear polypropylene 497 * √

0 2 ml microcentrifuge tube - quality 0 2 ml

2.0mL plastic Microcentrifuge Centrifuge Tube for Laboratory Name REF. Material Volume capacity RCF Qty in bag Qty in case Detailed information Microcentrifuge HRK-8100 PP 1.5ml 17800×g 500 10000 Snao Cap with safety lock, Toothed Made of High ..

T-Vector pMD20 - Takara Bio

2022-1-25 · T-Vector pMD20 is a linearized vector with a single 3’-terminal thymidine at both ends. The T-overhang ends at the cloning site improve the Gently flick the microcentrifuge tube to mix and then place it on ice bath for 30 minutes. 5. Heat-shock the cells for 45 seconds in a water bath at 42℃.

SuperCos 1 Cosmid Vector Kit - Agilent

2016-8-30 · 3. Aliquot into sterile microcentrifuge tubes (1 ml/tube) and store at –20°C for 1–2 years or –80°C for more than 2 years. PREPARATION OF VECTOR DNA Digestion of Vector DNA with Xba I 1. Digest 25 μg of the SuperCos 1 cosmid vector (1.0 μg/μl) with 9 U/μg of Xba I enzyme in a total volume of 200 μl at standard buffer

Microcentrifuge Tubes | Thermo Fisher Scientific

The 0.25mL and 0.4mL Thermo Scientific™ Specialty Microcentrifuge Tubes are ideal for small volume protocols when a standard volume microcentrifuge tube is not required. The 1.2mL microtiter tubes offer additional volume capacity for sample dilution. These are available in a pre-racked 8 x 12 format and can be autoclaved, sealed and frozen

0.5 ml Microcentrifuge Tube

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Microcentrifuge Tube Racks at Thomas Scientific

IsoFreeze Microcentrifuge Tube Racks. …enables you to see how cold your samples are at a glance. IsoFreeze® color change racks undergo a dramatic change to a contrasting color at 7ºC. Dimensions: 146 × 105 × 70 mm. Tube capacity: 24 x 0.5, 1.5, 2.0 mL snap or screw-cap style tubes. Rack comes with lid.

SuperCos 1 Cosmid Vector Kit - Agilent

2016-8-30 · 3. Aliquot into sterile microcentrifuge tubes (1 ml/tube) and store at –20°C for 1–2 years or –80°C for more than 2 years. PREPARATION OF VECTOR DNA Digestion of Vector DNA with Xba I 1. Digest 25 μg of the SuperCos 1 cosmid vector (1.0 μg/μl) with 9 U/μg of Xba I enzyme in a total volume of 200 μl at standard buffer

Ligation Protocol with T4 DNA Ligase (M0202) - NEB

Set up the following reaction in a microcentrifuge tube on ice. (T4 DNA Ligase should be added last. Note that the table shows a ligation using a molar ratio of 1:3 vector to insert for the indied DNA sizes.) Use NEBioCalculator to calculate molar ratios. * The T4 DNA Ligase Buffer should be thawed and resuspended at room temperature.

Blue Sup Brown Pellet Clip Art - Vector Clip …

You Can Free Download Blue Sup Brown Pellet Clip Art Vector Clip Microcentrifuge Tube With Pellet Png,Sup Icon Png (355x594). The Image''s Backgroud is Transparent And In PNG (Portable Network Graphics) Format. We Also Prepare Other Similar Super Mario Logo,Superman Logo,Superman Png,Supernatural Logo,Support Icon Cliparts For You.

Microcentrifuge Tube

: > > Sigma-Aldrich > Microcentrifuge Tube Microcentrifuge Tube HS4323K pack of 500 ea, pack of 500 ea, tube capacity 1.5 mL pack of 500 ea, tube capacity 1.5 mL 272.77 * √